KLM will cease its three times-daily feeder flights between Liverpool and Amsterdam from next March.
The Dutch carrier commenced flights between the two cities in 2009, the first time it had offered such services since the Second World War.
The flights will cease on March 25, 2012, with KLM’s UK General Manager Henri Hourcade blaming the "continued economic downturn for the route's demise.
"[It] has meant that as a company we have had to make some tough decisions across our global network and unfortunately Liverpool is a casualty of that process," he said.
Commenting on the news, Easyjet (which serves the Liverpool-Amsterdam route up to three times-daily) said:
“Easyjet has been proud to serve the people of Liverpool since 1996. We are proud that we were providing a regular service from Liverpool to Amsterdam before the airport decided to work with KLM.
"And we are proud to state that we continue to provide a regular service to Amsterdam both now and once KLM has gone.”