Flybe is cancelling its London City to Dublin route on May 31.
The regional airline, which launched the route five months ago, blamed weak passenger numbers for its decision.
It follows last week announcement that it’s suspending its London Southend to Dublin route and last month, it suspended its London City to Inverness service.
Paul Simmons, Flybe CCO, told City AM: "Despite our competitive pricing structure, overall passenger numbers are below our projections and there are no signs of this situation changing, which has led to us taking this decision.
"Flybe has a disciplined approach to the routes it operates, which means we continually review our network to ensure that we offer the right choice of destinations for our customers."