Three former British Airways bosses and its present global director of sales appeared in Southwark Crown Court yesterday.
The men face price-fixing charges brought by the Office of Fair Trading.
All four stand accused of colluding with rival carrier Virgin Airlines to set fuel surcharges on ticket prices between 2004 and 2006.
BA's global sales and marketing director Andrew Crawley, ex-commercial director Martin George, former head of communications Iain Burns and ex-head of sales in the UK and Ireland Alan Burnett, have pleaded not guilty.
The case is likely to start on Wednesday morning after jury selection is finalised and is expected to last about three months.
Virgin Atlantic and its staff are immune from prosecution having admitted the collusion to the OFT.
BA was fined £120 million by the OFT and £65 million by the US Department of Justice.
Both airlines were ordered to pay back overpriced surcharges to customers, costing each hundreds of millions of pounds.